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Date Published: 28/02/2026
Abstract Views: 61
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DOI: 10.56535/jmpm.v51i2.1738
Issue
Vol. 51 No. 2 (2026): JOURNAL OF MILITARY PHARMACO-MEDICINE No.2 - 2026
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Articles
How to Cite
Nguyen, T. D., Phan, M. H., Huynh, T. P., Thai, H. L., & Phan Van, H. N. (2026). SYNTHESIS OF RIFAMPICIN IMPURITIES A AND B. Journal of Military Pharmaco-medicine, 51(2), 25-34. https://doi.org/10.56535/jmpm.v51i2.1738

SYNTHESIS OF RIFAMPICIN IMPURITIES A AND B

Tien Dung Nguyen1, Minh Hoang Phan1, Tan Phat Huynh1, Hien Luong Thai1, Ho Nam Phan Van1,
1 Ho Chi Minh City University of Medicine and Pharmacy

Main Article Content

Abstract

Objectives: To synthesize impurity A (rifampicin quinone) and impurity B (rifampicin N-oxide) of rifampicin with high purity, suitable for use as reference materials. Methods: Impurity A and B were synthesized using oxidizing rifampicin with different oxidizing agents and purified using solid-phase extraction (impurity A) or flash column chromatography (impurity B). The purified products were structurally confirmed using spectroscopic techniques, including IR, MS, and NMR. Results: A total of 1,600mg of impurity A and 466.5mg of impurity B were synthesized with high purity, with yields of 56.46% and 16.10%, respectively. Conclusion: Impurity A and B of rifampicin were successfully synthesized, providing materials for establishing rifampicin impurity reference standards and contributing to the development of a national impurity reference database for quality control of related substances in drug products.

Keywords

Rifampicin, Rifampicin quinone, Rifampicin N-oxide

Article Details

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